為提高菌體產量,獲取更多目的蛋白產物,采用正交法和響應面法,優化益生菌枯草芽孢桿菌表達菌株Asr的發酵工藝。結果顯示影響菌體產量最主要的3個因素為酵母浸粉、MgSO4和CaCl2。最佳發酵培養基配方為：蔗糖20 g/L、蛋白胨10 g/L、酵母浸粉8.65 g/L、K2HPO4 3.0 g/L、MgSO4 0.27 g/L、CaCl2 0.53 g/L。菌體產量較優化前提高了1.76倍,平均達到了3.25×108 CFU/mL?；趦灮蟮呐囵B基,通過3 L發酵罐單因素試驗優化發酵條件：最佳培養溫度37 ℃、初始pH值7.0、接種量10%、攪拌轉速為250 r/min?？莶菅挎邨U菌Asr最高產量達到7.30×108 CFU/mL,試驗結果為后續的發酵罐擴大培養提高生物產量奠定了基礎。
In order to increase bacterial yield and obtain more target protein products, orthogonal method and response surface method were used to optimize the fermentation process of probiotic Bacillus subtilis expression strain Asr. The results showed that the three main factors affecting bacterial yield were leaching yeast powder, MgSO4 and CaCl2. The optimized fermentation medium formulation was: sucrose 20 g/L, peptone 10 g/L, leaching yeast powder 8.65 g/L, K2HPO4 3.0 g/L, MgSO4 0.27 g/L, CaCl2 0.53 g/L. The bacterial yield increased by 1.76-fold compared with that before optimization, with an average of 3.25×108 CFU/mL. After optimum of culture media, the fermentation conditions were optimized by single factor test in 3 L fermentor: the optimal temperature was 37 ℃, the initial pH value was 7.0, the inoculation volume was 10%, and the stirring speed was 250 r/min. The highest yield of Bacillus subtilis Asr reached 7.30×108 CFU/mL, and the test results laid a foundation for the subsequent expansion of fermentation tanks to increase biomass production.